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FIG. 3. AYF, Receiver operating characteristic curves for serum <t>miRNAs</t> of sepsis nonsurvivors (n = 24) and survivors (n = 32). G, These six miRNAs were confirmed by qRT-PCR. Areas under the ROC curves are also shown. H, Conjoint analysis of the six miRNAs.
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FIG. 3. AYF, Receiver operating characteristic curves for serum <t>miRNAs</t> of sepsis nonsurvivors (n = 24) and survivors (n = 32). G, These six miRNAs were confirmed by qRT-PCR. Areas under the ROC curves are also shown. H, Conjoint analysis of the six miRNAs.
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FIG. 3. AYF, Receiver operating characteristic curves for serum <t>miRNAs</t> of sepsis nonsurvivors (n = 24) and survivors (n = 32). G, These six miRNAs were confirmed by qRT-PCR. Areas under the ROC curves are also shown. H, Conjoint analysis of the six miRNAs.
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FIG. 3. AYF, Receiver operating characteristic curves for serum <t>miRNAs</t> of sepsis nonsurvivors (n = 24) and survivors (n = 32). G, These six miRNAs were confirmed by qRT-PCR. Areas under the ROC curves are also shown. H, Conjoint analysis of the six miRNAs.
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FIG. 3. AYF, Receiver operating characteristic curves for serum <t>miRNAs</t> of sepsis nonsurvivors (n = 24) and survivors (n = 32). G, These six miRNAs were confirmed by qRT-PCR. Areas under the ROC curves are also shown. H, Conjoint analysis of the six miRNAs.
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Expression profiles of Tartary buckwheat <t>miRNA</t> biosynthesis genes in different organs and during seed development. The different colors represent the expression abundance of the genes
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Expression profiles of Tartary buckwheat <t>miRNA</t> biosynthesis genes in different organs and during seed development. The different colors represent the expression abundance of the genes
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FIG. 3. AYF, Receiver operating characteristic curves for serum miRNAs of sepsis nonsurvivors (n = 24) and survivors (n = 32). G, These six miRNAs were confirmed by qRT-PCR. Areas under the ROC curves are also shown. H, Conjoint analysis of the six miRNAs.

Journal: Shock

Article Title: Characterization and Identification of Novel Serum MicroRNAs in Sepsis Patients With Different Outcomes

doi: 10.1097/shk.0b013e3182940cb8

Figure Lengend Snippet: FIG. 3. AYF, Receiver operating characteristic curves for serum miRNAs of sepsis nonsurvivors (n = 24) and survivors (n = 32). G, These six miRNAs were confirmed by qRT-PCR. Areas under the ROC curves are also shown. H, Conjoint analysis of the six miRNAs.

Article Snippet: A miRNA first-strand cDNA synthesis kit and miRcute miRNA qPCR detection kit (SYBR) (Tiangen Biotech Company, Beijing, China) were used for qRT-PCR validation of the novel predicted miRNAs.

Techniques: Quantitative RT-PCR

Expression profiles of Tartary buckwheat miRNA biosynthesis genes in different organs and during seed development. The different colors represent the expression abundance of the genes

Journal: BMC Plant Biology

Article Title: Integrated microRNA and transcriptome profiling reveal key miRNA-mRNA interaction pairs associated with seed development in Tartary buckwheat ( Fagopyrum tataricum )

doi: 10.1186/s12870-021-02914-w

Figure Lengend Snippet: Expression profiles of Tartary buckwheat miRNA biosynthesis genes in different organs and during seed development. The different colors represent the expression abundance of the genes

Article Snippet: The qRT-PCR was performed on a CFX96 Real-time System (BIO-RAD, USA) using the miRcute Plus miRNA qPCR Kit (SYBR Green) (Tiangen, Beijing, China).

Techniques: Expressing

DEMs during Tartary buckwheat seed development. a Venn diagram represented the overlap of DEMs among the comparisons. b Number of DEMs in the comparisons of S1 vs. S2, S1 vs. S3, and S2 vs. S3. Green and yellow bars represented the number of up- or down-regulated genes, respectively. c Expression heat map of DEMs. The different colors represent the expression abundance of the miRNAs

Journal: BMC Plant Biology

Article Title: Integrated microRNA and transcriptome profiling reveal key miRNA-mRNA interaction pairs associated with seed development in Tartary buckwheat ( Fagopyrum tataricum )

doi: 10.1186/s12870-021-02914-w

Figure Lengend Snippet: DEMs during Tartary buckwheat seed development. a Venn diagram represented the overlap of DEMs among the comparisons. b Number of DEMs in the comparisons of S1 vs. S2, S1 vs. S3, and S2 vs. S3. Green and yellow bars represented the number of up- or down-regulated genes, respectively. c Expression heat map of DEMs. The different colors represent the expression abundance of the miRNAs

Article Snippet: The qRT-PCR was performed on a CFX96 Real-time System (BIO-RAD, USA) using the miRcute Plus miRNA qPCR Kit (SYBR Green) (Tiangen, Beijing, China).

Techniques: Expressing

Identified key  miRNA-mRNA  interaction pairs for tartary buckwheat seed development

Journal: BMC Plant Biology

Article Title: Integrated microRNA and transcriptome profiling reveal key miRNA-mRNA interaction pairs associated with seed development in Tartary buckwheat ( Fagopyrum tataricum )

doi: 10.1186/s12870-021-02914-w

Figure Lengend Snippet: Identified key miRNA-mRNA interaction pairs for tartary buckwheat seed development

Article Snippet: The qRT-PCR was performed on a CFX96 Real-time System (BIO-RAD, USA) using the miRcute Plus miRNA qPCR Kit (SYBR Green) (Tiangen, Beijing, China).

Techniques: Binding Assay, Cell Differentiation, Starch, Transduction, Inhibition, Phospho-proteomics, Membrane

miRNA-mRNA interaction pairs showed significant negative correlation (R ≥ 0.5, P < 0.05) of expression during Tartary buckwheat seed development. Left: Heat map of DEMs. Right: Heat map of the differentially expressed target mRNAs of DEMs. The different colors represent the expression abundance of the miRNAs and their corresponding target mRNAs

Journal: BMC Plant Biology

Article Title: Integrated microRNA and transcriptome profiling reveal key miRNA-mRNA interaction pairs associated with seed development in Tartary buckwheat ( Fagopyrum tataricum )

doi: 10.1186/s12870-021-02914-w

Figure Lengend Snippet: miRNA-mRNA interaction pairs showed significant negative correlation (R ≥ 0.5, P < 0.05) of expression during Tartary buckwheat seed development. Left: Heat map of DEMs. Right: Heat map of the differentially expressed target mRNAs of DEMs. The different colors represent the expression abundance of the miRNAs and their corresponding target mRNAs

Article Snippet: The qRT-PCR was performed on a CFX96 Real-time System (BIO-RAD, USA) using the miRcute Plus miRNA qPCR Kit (SYBR Green) (Tiangen, Beijing, China).

Techniques: Expressing

Quantitative real-time PCR validation of six identified key miRNA-mRNA interaction pairs for Tartary buckwheat seed development. Green and blue bars represented the miRNAs and their corresponding target mRNAs, respectively. The error bar represents the error values of three biological replicates

Journal: BMC Plant Biology

Article Title: Integrated microRNA and transcriptome profiling reveal key miRNA-mRNA interaction pairs associated with seed development in Tartary buckwheat ( Fagopyrum tataricum )

doi: 10.1186/s12870-021-02914-w

Figure Lengend Snippet: Quantitative real-time PCR validation of six identified key miRNA-mRNA interaction pairs for Tartary buckwheat seed development. Green and blue bars represented the miRNAs and their corresponding target mRNAs, respectively. The error bar represents the error values of three biological replicates

Article Snippet: The qRT-PCR was performed on a CFX96 Real-time System (BIO-RAD, USA) using the miRcute Plus miRNA qPCR Kit (SYBR Green) (Tiangen, Beijing, China).

Techniques: Real-time Polymerase Chain Reaction, Biomarker Discovery

5′ RLM-RACE verification of six identified key miRNA-mRNA interaction pairs for Tartary buckwheat seed development. The numbers above sequences indicate the detected cleavage site of independent clones

Journal: BMC Plant Biology

Article Title: Integrated microRNA and transcriptome profiling reveal key miRNA-mRNA interaction pairs associated with seed development in Tartary buckwheat ( Fagopyrum tataricum )

doi: 10.1186/s12870-021-02914-w

Figure Lengend Snippet: 5′ RLM-RACE verification of six identified key miRNA-mRNA interaction pairs for Tartary buckwheat seed development. The numbers above sequences indicate the detected cleavage site of independent clones

Article Snippet: The qRT-PCR was performed on a CFX96 Real-time System (BIO-RAD, USA) using the miRcute Plus miRNA qPCR Kit (SYBR Green) (Tiangen, Beijing, China).

Techniques: Clone Assay